A Novel Plasmid-Based Microarray Screen Identifies Suppressors of rrp6 in Saccharomyces cerevisiae †
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چکیده
A Novel Plasmid-Based Microarray Screen Identifies Suppressors of rrp6 in Saccharomyces cerevisiae † Katharine Abruzzi,§ Sylvia Denome,§ Jens Raabjerg Olsen,‡ Jannie Assenholt, Line Lindegaard Haaning, Torben Heick Jensen, and Michael Rosbash* Howard Hughes Medical Institute and Department of Biology, Brandeis University, Waltham, Massachusetts 02454, and Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, Aarhus University, C. F. Møllers Alle, Building 130, 8000 Aarhus C, Denmark
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A novel plasmid-based microarray screen identifies suppressors of rrp6Delta in Saccharomyces cerevisiae.
Genetic screens in Saccharomyces cerevisiae provide novel information about interacting genes and pathways. We screened for high-copy-number suppressors of a strain with the gene encoding the nuclear exosome component Rrp6p deleted, with either a traditional plate screen for suppressors of rrp6Delta temperature sensitivity or a novel microarray enhancer/suppressor screening (MES) strategy. MES ...
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